The balance of supply and demand of ecosystem services is an important part of regional sustainable development and an important indicator to measure whether regional socio-economic and natural development can be coordinated. Taking the demonstration zone of green and integrated ecological development of the Yangtze River Delta as an example, this paper used the InVEST, CASA and Thornthwaite Memoriai models to measure the regional supply and demand of water yield, carbon storage, and food production services in 2000, 2005, 2010, 2015 and 2020 from the perspective of the water-energy-food nexus. The Gini coefficient, supply and demand index and correlation coefficient were introduced to explore the spatial characteristics of regional ecosystem service supply and demand. The results revealed that (1) from 2000 to 2020, except for the supply of food production service, which fluctuated down, the supply of other ecosystem services showed a fluctuating upward trend. In terms of demand, all the ecosystem services showed an upward trend, with the largest increase in water and carbon storage demand is Yaozhuang Township, Jiashan County, while food demand, showed an overall upward trend during the study period,except for Liantang Township,Qingpu District. (2) In terms of spatial distribution, the southeast and southwest regions had high values in the supply of water yield and food production services, while the high value area of carbon storage was concentrated in Taoyuan Township, Wujiang District, and the high value area of demand for various ecosystem services was mainly distributed in a circular pattern around towns with high population density. (3)The Gini coefficient of each ecosystem service in the demonstration area was less than 0.3, the spatial distribution of ecosystem services in the region was in equilibrium, the supply and demand indices of all kinds of ecosystem services were decreasing, and the water yield service of Jiaxing had turned from an obvious surplus to a deficit state. In terms of spatial distribution, water yield service in Jiashan had been in surplus from 2000-2015, and the surplus area tended to spread. Three ecosystem services were in short supply in densely populated areas, and the difference between urban and rural supply and demand matching was very obvious.(4) On the supply side, water yield and food production showed a significantly synergistic relationship, and the trade-off/synergistic between water yield and carbon storage, as well as carbon storage and food production were insignificant. On the demand side, the three ecosystem services showed a two-by-two synergistic relationship. By exploring the spatial and temporal distribution of ecosystem services supply and demand in the demonstration area, this study is conducive to the construction of a regional ecological security pattern and can provide a reference for the realization of ecosystem service supply and demand in the Yangtze River Delta region.