Abstract:Disturbance is an important factor affecting forest restoration and ecological succession. It changes plant community composition and microenvironment, and then affects carbon distribution and carbon sequestration potential of forest ecosystem. In this study, we selected disturbance sequence secondary forests, i.e., the near-mature secondary forest (NS, 56 years) formed by natural succession after clear-cutting of the mixed-broadleaved Korean pine forest, the half-mature secondary forest (MS, 25 years) formed by natural succession after clear cutting of NS, and disturbed young secondary forest (YD, 15 years) formed by natural succession after clear cutting of NS and the soil has been disturbed for 15 years. We used the inventory and biometry-based measurement to examine the carbon density and its distribution. Our objectives were to quantify the effect of disturbance types and vegetation restoration on the carbon density and its distribution pattern of the temperate secondary forests in Northeast China. The results showed that the soil organic carbon content ([SOC]) of different soil layers (0-50 cm) varied from 10.46 to 29.27 mg/g, 6.37 to 108.40 mg/g, 5.21 to 114.34 mg/g in YD, MS, and NS, respectively. And the[SOC] decreased significantly with the deepening of soil layer. There were significant differences in the topsoil (0-20 cm)[SOC] among the sequence secondary forests (P<0.01), but no significant difference in subsoil. The[SOC] was negatively correlated with bulk density. The topsoil organic carbon density accounted for more than 50% of the total soil organic carbon density. The topsoil SOC density of YD (30.91 t/hm2) was significantly lower than that of MS (54.09 t/hm2) and NS (55.14 t/hm2). The carbon density of vegetation, soil (0-100 cm), and ecosystem varied from 45.63 to 98.97 t/hm2, 62.09 to 91.67 t/hm2, and 107.72 to 181.26 t/hm2 in the three secondary forests, respectively. The carbon density of vegetation increased with the stand age, but the carbon density change trend of the tree species in different succession periods was not consistent. The carbon density of the pioneer tree species ((37.47±2.64) t/hm2) and shrub species ((0.85±0.48) t/hm2) was not significantly different among the three forest types, while the carbon density of the later species was significantly different among the three secondary forests, and varied from 4.05 t/hm2 to 62.48 t/hm2. The mean annual increment (MAI) of carbon density decreased from 3.04 t hm-2 a-1 in YD to 1.77 t hm-2 a-1 in NS. The difference was mainly due to the contributions of pioneer species during succession, while MAI of later species and shrub species did not differ significantly among secondary stands. These findings indicated that the soil disturbance more than ten years changed its microenvironment and bulk density, resulting in the topsoil organic carbon loss of temperate forest in Northeast China. However, the aboveground vegetation cutting disturbance had no significant effect on soil organic carbon. In the process of vegetation restoration, pioneer species promoted short-term carbon fixation, while later species determined vegetation carbon density and carbon sink potential. This provides data support and theoretical basis for the evaluation and estimation of carbon sequestration and its management in temperate forest ecosystems in northeast China.