The effective and systematic identification of the regional ecosystem service clusters and structural patterns is prerequisite and foundation for revealing the objective laws of human-earth system. Based on the results of climatic zoning, agricultural natural zoning, major functional zoning division, and three-level watershed unit in China, the land area regionalization of Guangdong Province was carried out from top to bottom following the rules of unity, relative consistency, regional conjunction and the principle of maintaining the integrity of natural and administrative boundaries as far as possible to maximize the integrity of watershed units and ecosystems. The values and z-score of 12 typical ecosystem services such as grain supply, raw material production, and water resource supply were calculated according to the zoning units. Finally, the K-means cluster method was applied to identify ecosystem service clusters and patterns. The results are as follows:the study area covered three natural zones, namely the middle subtropical zone, the south subtropical zone and the marginal tropical zone, and was further divided into nine economic zones with a total of 34 watershed units, forming a three-level zoning system of "3+9+34". Five types of ecosystem service cluster structure patterns were identified to objectively demonstrate the symmetrical spatial distribution characteristics of terrestrial ecosystem services in Guangdong Province with the Pearl River Delta as the core, which include hydrologic regulation and protection type, soil and water conservation regulation type, ecological conservation park type, ecological livable and harmonious type, and comprehensive coordinated supply type. Affected by terrain, landform and other natural geographical environment as well as human activities such as land use, each region of ecosystem service cluster type has its own unique terrain and geographical characteristics and undertakes different ecological service functions. Based on the research results of recognition and analysis of ecosystem service cluster patterns under region division, we made some discussions on proposal for differentiated ecosystem control measures based on ecosystem service clusters, which could provide a reference for regional sustainable development.