拟南芥与叶际微生物组的互作遗传机制——基于网络作图理论
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国家自然科学基金(31971398);北京林业大学大学生创新计划(S202110022045,S202210022047,X202210022062)


Genetic mechanism of interaction between Arabidopsis thaliana and the phyllosphere microbiome by network mapping
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National Natural Science Foundation of China(31971398);Undergraduate innovation program of Beijing Forestry University (S202110022045、S202210022047、X202210022062)

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    摘要:

    叶际微生物组对植物的生长发育至关重要,但植物与其定殖微生物组相互作用机制尚不明确。目前植物与微生物互作研究多集中于根际微生物组,对叶际微生物组的研究较少,且这些研究未能从微生物互作的角度探究植物与微生物的相互作用机理。基于网络作图理论,将拟南芥基因组SNP (Single Nucleotide Polymorphisms)分子标记数据与微生物组网络特征值相关联,挖掘影响叶际微生物组网络结构的枢纽基因,以探究拟南芥塑造叶际微生物组网络结构的遗传机制。通过对188株拟南芥及其叶际微生物组数据的分析,识别出四种关系下的中心节点微生物,筛选到622个显著SNP位点。进一步构建了贝叶斯遗传网络,获得26个枢纽基因,这些基因可能参与了植物抗病、激素分泌和生长发育相关的分子途径。本研究从全基因组角度探究植物调控自身微生物组的遗传机制,揭示植物与微生物组如何互作促进植物健康,将为精准分子育种提供理论基础和遗传资源,并为合成菌群用于创制新型菌剂提供数据支持,具有重要的科学意义和应用价值。

    Abstract:

    The phyllosphere microbiome is critical for plant growth and development, but the mechanism by which plants interact with their colonizing microbiome remains elusive. At present, most studies on the interaction between plants and microorganisms focus on the rhizosphere microbiome rather than those of phyllosphere. Moreover, these studies failed to disentangle the mechanism of plant-microbe interactions from the perspective of microbial interaction. In this study, based on the network mapping theory, we correlated the Arabidopsis thaliana genome SNP(Single Nucleotide Polymorphisms) molecular marker data with the microbiome network indices, mined the pivot genes that affected the phyllosphere microbiome network structure to explore the genetic mechanisms how A.thaliana shaped the network structure of phyllosphere microbiome. We analyzed 188 A.thaliana and their phyllosphere microbiome data, identified the hub microbes under four relationships, and screened 622 significant SNP loci. By Bayesian genetic network, 26 hub genes were excavated, which involved in molecular pathways related to plant disease resistance, hormone secretion, and growth and development. This study explored the genetic mechanism of plant regulation of its own microbiome from a genome-wide perspective and revealed how plants and microbiome interacted to promote plant health. It will provide theoretical basis and genetic resources for precision molecular breeding, and support the use of synthetic communities to create new bacterial agents, containing important scientific significance and application value.

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王浩臣,蔡雨皓,谭静怡,王梓源,谭惠文,李凯航,程可心,杨艳,金一,何晓青.拟南芥与叶际微生物组的互作遗传机制——基于网络作图理论.生态学报,2023,43(19):8011~8024

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