Ozone(O₃) has become the primary summer pollutant in some large cities in China. Its strong phytotoxicity posed a severe threat to agricultural security. Soybeans are considered one of the most sensitive crops to O₃ contamination, and high levels of ozone exposure can lead to soybean yield reduction. O₃ pollution often occurs on hot and sunny days and is intermittent and cumulative. So under natural conditions, intermittent ozone exposure is the primary exposure mode for soybeans. Still, most previous studies focused on the effects of long-term O₃ stress on plant physiological processes at the leaf scale. In contrast, few studies have investigated the effects of intermittent exposure on whole plant growth and photosynthetic physiological properties. In this study, we set two levels of O₃ concentration control check (ambient O₃ concentration) and treatment (above ambient 80 nL/L) based on open-top chambers. The fumigation experiments were conducted in two sessions of 5 days each. The 12 pots of soybeans were divided into two groups of six replicates each. Before and after intermittent O₃ exposure, the photosynthesis of the soybeans population was measured by a custom-made dynamic flux chamber. After the soybean was harvested, indicators such as biomass and yield per plant were counted. At last, we investigated the response of soybean leaf population photosynthesis as well as yield to intermittent O₃ exposure when the ambient ozone concentration increases by 80 nL/L. The results showed that (1) The effect of intermittent O₃ exposure was cumulative and reversible, and the net photosynthetic rate of soybean plants was reduced at low O₃ exposure doses (AOT40 ≤ 2.47 μL L^-1 h^-1), which was not significantly different from the control group. When AOT40 was less than 5.35 μL L^-1 h^-1, the net photosynthetic rate of soybean plants was reduced considerably. While O₃ stress disappeared, the net photosynthetic rate of soybean plants gradually rebounded and eventually recovered. (2) Different photosynthetic parameters were differentially sensitive to intermittent ozone exposure. The maximum net photosynthetic rate was the most sensitive. It was considerably reduced under low AOT40, and the recovery time was longer. (3) The net photosynthetic rate was lower and recovered faster after the secondary O₃ exposure, suggesting that intermittent O₃ exposure might increase the tolerance threshold of soybean. (4) When AOT40 was below 5.35 μL L^-1 h^-1, there was no significant effect on soybean yield, which indicated that soybean yield reduction thresholds were higher under intermittent ozone exposure conditions.