Constructing an ecological network is an important means to maintain landscape connectivity and improve ecosystem services, including carbon sequestration. To better respond to the goal of carbon sequestration and its policy requirements, it is necessary to incorporate spatial carbon emission factors into the framework of regionally ecological network analysis. Taking the Hohhot-Baotou-Ordos-Yulin urban agglomeration with high energy consumption, high carbon emissions and fragile ecology as the study area, the ecological sources were identified based on ecosystem services and connectivity analysis, and ecological corridors were extracted by combining circuit theory and the gravity model to obtain the regional landscape ecological pattern. The grid-scale carbon emission spatial pattern of the urban agglomeration was simulated by obtaining nighttime light data, spatially superimposed with the landscape ecological pattern to supplement the "ecology-carbon emission" coupling nodes and planning corridors, and then constructing the urban agglomeration ecological network considering the regional carbon emission spatial pattern. The results showed that 1) the regionally ecological network constructed by only considering ecological factors had insufficient connectivity between ecological sources on the east and west sides, and it showed a "central-blocking" distribution. 2) Regional carbon emissions had obviously spatial characteristics, and high-value areas appeared in the central mineral resource development zones and the major urban areas with significantly anthropogenic ecological disturbances. 3) By coupling the regional carbon emission pattern, 23 "ecology-carbon emission" coupling nodes and 67 planned corridors were added, the ecological network structure changed from tree-like to honeycomb-like, and network circuitry, line-to-node ratio, and network connectivity increased by 92%, 30.48%, and 27.45%, respectively. Therefore, the improved ecological network construction method coupling spatial carbon emission factors is of great significance for improving the multiple functions and stability of the ecological network, as well as promoting ecological protection and restoration aimed at increasing carbon sequestration and sinks.