Abstract:Snow cover is an important way to increase soil moisture content in temperate desert, which can provide abundant water during the freezing-thawing period. Changes in spatial and temporal patterns of snow depth under global changes in temperate deserts affect greenhouse gas flux in desert ecosystems. Biological soil crusts (biocrusts) play crucial role in stability of structure and function of desert ecosystem, while they are sensitive to environmental changes, especially to precipitation changes. However, few studies on greenhouse gas fluxes were found in desert biocrusts during freezing-thawing period under snow treatments. CH4 is an important greenhouse gas. In this study, two types of biocrusts, moss and lichen crusts were selected, and bare sand was as control. Three snow cover treatments, including snow addition, natural snow (control) and snow reduction were conducted. We aimed to explore the variation in CH4 flux in 2019 and 2021 during freezing-thawing period based on static chamber-gas chromatography. In general, snow treatment and crust types had no significant effects on CH4 flux. Desert biocrusts generally absorbed CH4 in this study, and were important "sinks" of CH4 in desert ecosystems. The absorption rates of CH4 for the two types of crusts and bare sand were lichen crusts (-10.12 μg m-2 h-1) > bare sand (-1.73μg m-2 h-1) > moss crusts (-1.69 μg m-2 h-1). CH4 fluxes were generally not significantly correlated with soil temperature and water content at 5 cm depth, and had weak response to other soil physicochemical factors. The results showed that snow cover treatments had no significant effects on CH4 flux in biocrusts during freezing-thawing period, and the change in snow cover in temperate desert may have few effects on CH4 production and absorption in soil under the condition of global change.