Habitat degradation is a major threat to Tachypleus tridentatus (T. tridentatus). In order to distinguish the effects of human activities on habitat degradation of juvenile T. tridentatus, the abundance of juvenile T. tridentatus in the intertidal zone was investigated by random sampling method at 2014, 2017 and 2020, and the remote sensing images with resolution of 30 m were obtained in corresponding years. With the help of GIS spatial analysis and landscape pattern analysis and the human disturbance index (HDI) model, the effects of human disturbance on the landscape pattern and population abundance of juvenile T. tridentatus in the intertidal zone of Beibu Gulf were quantitatively evaluated. The results show that:(1) The suitable habitat of juvenile limulus in the intertidal zone of the Beibu Gulf deteriorated seriously. The area of undisturbed landscape as almost undisturbed by human decreased gradually, most of which were transformed into aquaculture. Adversely, the landscapes that might pose the greatest threat to the survival of horseshoe crabs are residential areas and port terminals. The increasing area of these unsuitable landscapes would cause the fragmentation of suitable habitats and reduce the quality of ecological environment, which is not conducive to the survival of juvenile horseshoe crabs. (2) The anthropogenic interference in the intertidal zone of Beibu Gulf was gradually strengthened. The area of HDI with undisturbed level was reduced, and the HDI of most areas in the intertidal zone showed an increasing trend. (3) The HDI was significantly negatively correlated with the population abundance of juvenile T. tridentatus. The abundance of juvenile T. tridentatus decreased with the increase of HDI. This result suggests that the high-value patches of HDI were not suitable for the survival of juvenile T. tridentatus. Based on the dynamic change and spatial distribution of HDI index, the optimization of habitat landscape pattern, the construction of protected areas and the site selection of artificial discharge and proliferation will be scientifically and effectively guided.