Even though inland freshwater waterbodies are important atmospheric N₂O sources, but current N₂O emission data from those waterbodies are still limited. Furthermore, it is unclear about N₂O spatiotemporal emission characters in different urban waterbodies and corresponding drivers. This study applied floating chambers to in-situ measure N₂O emission fluxes in typical urban waterbodies including reservoirs, rivers, aquaculture ponds and scenic ponds of Nanjing starting from May of 2020 to April of 2021. Furthermore, physical and chemical water parameters were also measured to elucidate main N₂O emission drivers. The result revealed that all types of urban waterbodies generally showed as emission sources. Rivers and aquaculture ponds had the largest N₂O flux, with average values of (503±1236) μg m^-2 d^-1 and (508±797) μg m^-2 d^-1, respectively, following by scenic ponds ((179±989) μg m^-2 d^-1), while reservoirs only showed as a weak N₂O emission source ((54±212) μg m^-2 d^-1). N₂O emissions from all waterbodies showed the seasonal pattern with higher N₂O fluxes in summer, which is especially pronounced in rivers and aquaculture ponds (P<0.01). The annual N₂O flux positively correlated with temperature and negatively correlated with dissolved oxygen (DO) (P<0.01), respectively. However, the nitrogen input was shown as the key driver for N₂O emissions starting from May to September with temperature higher than 20℃. Therefore, controlling nitrogen input especially in the Summer season was an important measure for reducing N₂O emissions. Furthermore, ponds showed large differences in N₂O emissions resulting from different hydro-geochemical conditions and other factors. Monitoring of hydro-geochemical conditions and N₂O emission fluxes for small waterbodies should be strengthened in further studies, and specific drivers for N₂O emissions had to be explored. This study contributed to N₂O emission estimations in the city, and provided the basis for updating N₂O emission models.