Most studies on interspecific association using sample methods focused on the association and correlation at different levels of community, niche and interspecific association characteristics of the main species in community. However, the systematic study on interspecific association and community stability changes of species in community is insufficient. Using the data of two community surveys on 11.56 hm² deciduous broad-leaved forest in the Yaoluoping Nature Reserve (YLPNR) at 5-year intervals (2016 and 2021), the changes of species diversity, interspecific association and community stability were studied by some methods including the α diversity index, variance ratio (VR), Chi-square test (χ² test), Pearson's correlation coefficient test, Spearman's rank correlation coefficient test, and M Godron stability analysis. The results showed that:(1) the important value of dominant species in the community increased, the number of shrubs and small trees increased sharply, such as Rhododendron simsii, Lindera praecox and Meliosma flexuosa, and resulting in the increase of the cumulative important value. (2) The variance ratio increased from 1.35 to 2.87, indicating the significant increase in overall positive correlation. χ² test showed that the ratio of positive and negative correlated species pairs among major species increased from 0.97 to 1.25, indicating that positive correlated species pairs began to dominate. (3) Pearson correlation coefficient test and Spearman rank correlation coefficient test showed that the extremely significant and significant correlated species pairs increased from 132 and 160 to 152 and 226, respectively, and the interspecific correlation was enhanced. (4) The result of M. Godron stability measurement increased from 25.59/74.41 to 23.64/76.36, and the community stability enhanced. Interspecific association analysis and community stability measurement reflected the community succession stage in different forms. This study reveals that the interspecific association of the community in the deciduous broad-leaved forest of YLPNR has been strengthened, and the distribution of each species was closer and the correlation was enhanced, and the community was developing towards a stable stage in accordance with the environmental conditions in this area.