Ecological conservation redline is the bottom line and lifeline for safeguarding and maintaining nationally ecological security. The ledger of ecological conservation redline is an important foundation for the supervision of ecological conservation redline. In view of the supervision goal of "no reduction of ecological function, no reduction of area and no change of nature", this paper designs a ledger organization and management method integrating space account and form account, and studies the key technologies of the ledger construction including full life cycle supervision, all elements refinement supervision and spatiotemporal integration supervision. Meanwhile, taking Jingyuan County of Ningxia Hui Autonomous Region as an example, it constructed 24 books of redline supervision ledgers in 5 categories from 2010 to 2020 and analyzed the application of ledger. The results show that:(1) the percent of ecological conservation redline area in Jingyuan County is 51.07% and most of the nature reserves are included. (2) the forest land and grassland within the ecological conservation redline account for 89.55% and 9.86%, indicating that most of the redline areas are natural ecosystems. (3) the number of residential areas within the ecological conservation redline accounts for 12.76% of the whole county, and the density is very low. (4) from 2010 to 2019, the vegetation coverage in the growing season within the ecological conservation redline remained basically stable, and showed a positive correlation with rainfall. (5) the water conservation, water and soil conservation, wind prevention and sand fixation within the redline account for 77.39%, 67.72% and 61.43% of the whole county respectively, indicating that most of the important ecological functions in the ecological space are protected.