To study the formation mechanism and characteristics of topography induced microhabitats is of great significance for the restoration and conservation of biodiversity in loess hilly-gully region. Hourly temperatures were recorded by Ibutton electronic temperature recorders at different slope position and aspects in Chenjiawa, Ansai County, Shaanxi Province. In order to quantify the thermal buffering effect of microhabitat, the extreme temperature at 5 cm and 150 cm in different slope positions was compared with the temperature at the zonal habitat (hill top). Average temperature, extreme high and extreme low temperature were used to evaluate the non-synchronicity of topography induced microhabitat to regional temperature change, and temperature variation was used to measure the climatic stability of the microhabitat. In the hottest two weeks, the maximum buffer capacity of hill bottom on shady slope was 6℃, and the maximum buffer capacity of the middle and upper slope was 4.5℃ and 3.5℃, respectively. The maximum thermal buffering capacity of the three slope positions on the sunny side from top to bottom was 3.5℃, 3℃ and 4℃, respectively. From the chart analysis, the buffer effect of shady slope on extreme high temperature in summer was greater than sunny slope. On the sunny slope, the maximum buffer value of microhabitat at hill bottom, middle slope and upper slope was 4.5℃, 4℃ and 4℃ in the coldest two weeks. On the shady slope, the maximum buffer value of microhabitat at hill bottom, middle slope and upper slope could reach 3.5℃, 3℃ and 2℃. The buffering effect on extreme low temperature in winter was smaller on shady slope than sunny slope. There were significant differences between different slope positions in both thermal buffering and cold buffering (P<0.05), the buffering effects of both shady slope and sunny slope showed a ladder from bottom to top as:hill bottom > middle slope > upper slope. The daily temperature variation range of the sunny slope was larger than the shady slope, and upper slope > middle slope > hill bottom. This study quantifies the thermal buffering capacity of microhabitats and emphasizes the importance of microhabitats in regulating regional climate change, which can provide support for the prediction of future climate change model.