The rapid urbanization has significant impacts on vegetation and biodiversity within urban area. Studying the response of plant community characteristics to artificial disturbance could provide scientific basis for ecological restoration, biodiversity protection and rational development and utilization of vegetation in urban remnant habitat. Based on remote sensing interpretation and community ecological investigation and analysis, the effects of different artificial disturbance ways and intensity on the vegetation characteristics, community species composition and diversity of urban remnant mountains (URMs) in Guiyang, a typical karst multi-mountainous city, were analyzed. The results showed that:(1) the URMs fractional vegetation coverage (FVC) and net primary productivity (NPP) were the highest under the moderate disturbance intensity. The FVC and NPP values of the URMs under the disturbance way of reclamation were consistent. The FVC and NPP values of small and medium-sized URMs under the disturbance way of park utilization were completely inconsistent, and their NPP values were the lowest due to the serious disturbance of construction and hard activity sites. (2) There were significant differences in the average number of species and composition among URMs under different disturbance intensity and ways. On the whole, the order of the average number of species of URMs from large to small under different disturbance intensities was light disturbance, moderate disturbance and severe disturbance; the order of the average number of URMs species under different disturbance ways from large to small was human trampling, structures, engineering construction, park utilization, reclamation and engineering excavation; Deciduous trees and shrubs accounted for the highest proportion in URMs under severe disturbance, while evergreen shrubs and perennial herbs dominated URMs under moderate disturbance. (3) Under different disturbance intensities, the species diversity indices of different plant layers were low and there were significant differences. The Shannon-Wiener (H'), Simpson (D) and Margalef (R) indices of tree layer and shrub layer were the largest under moderate disturbance intensity, while those of herb layer were the smallest; Pielou evenness (Jh) indices of tree layer and herb layer were in the order of light disturbance, moderate disturbance and severe disturbance from high to low, while the Jh indices was completely opposite in shrub layer. (4) The diversity indices of tree layer varied greatly under different disturbance ways. The diversity indices of shrub layer and herb layer were significantly higher under the disturbance ways of human trampling and construction than that under the other disturbance ways, and were lower under the disturbance ways dominated by engineering excavation, and there were significant differences. In general, the light and moderate artificial disturbances did not significantly affect the plant species composition and diversity of URMs, and the moderate artificial disturbance promoted the plant species diversity to a certain extent, while the severe artificial disturbance had significantly negative effect on the plant community characteristics of URMs.