玉米和花生间作根际细菌群落结构与PICRUSt的功能预测
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国家自然科学基金项目(81973412);福建省自然科学基金项目(2021J01090);平潭科技研究院项目(PT2021008);福建农林大学杰出青年科研人才计划项目(XJQ201501)


Analysis of rhizosphere bacterial community structure and PICRUSt-predicted functional categories in maize and peanut intercropping systems
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The National Natural Science Foundation of China (81973412); The Natural Science Foundation of Fujian Province ( 2021J01090); Innovation Fund of Pingtan Institute of Science and Technology (PT2021008); Science and Technology Innovation Fund of Fujian Agriculture and Forestry University (Grant no. CXZX2020039A); The Outstanding Youth Scientific Fund of Fujian Agriculture and Forestry University (Grant No. XJQ201501)

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    摘要:

    禾本科/豆科作物间作能够促进作物生长与产量形成,而根际微生物组被认为与间作产量优势形成关系密切。将玉米和花生进行盆栽间作并设置以下三种根系分隔方式:塑料膜分隔(即全隔玉米与全隔花生)、尼龙网分隔(即网隔玉米与网隔花生)、无分隔处理(即无隔玉米与无隔花生),采用基于16S rRNA基因的高通量测序技术及PICRUSt功能预测分析不同模式下二者根际细菌群落结构与功能类群的变化,以期从土壤微生物组角度阐明玉米/花生间作产量优势形成的内在机制。结果显示:玉米/花生间作明显改变了二者根际细菌群落结构,无隔、网隔玉米的根际细菌群落结构更为相似并区别于全隔玉米,而网隔花生根际细菌群落结构明显区别于无隔、全隔花生。而且,无隔、网隔玉米根际放线菌门的相对丰度显著高于全隔玉米,网隔花生根际放线菌门、厚壁菌门的相对丰度显著高于全隔、无隔花生,而芽单胞菌门呈现相反的变化趋势。在玉米和花生根际,无隔、网隔处理高于全隔处理的OTU也均主要注释为放线菌门。共存网络分析发现,作为玉米根际细菌网络重要节点的StreptomycesActinomaduraArthrobacterBacillus等菌属其丰度变化表现为无隔、网隔处理高于全隔处理;类似地,花生根际StreptomycesActinomaduraArthrobacter等重要节点也呈现网隔处理高于无隔、全隔处理的变化趋势。最后,PICRUSt功能预测分析发现,玉米/花生间作显著提高了膜转运、碳水化合物代谢等功能类的相对丰度而降低了复制与修复等功能类的相对丰度。综上可见,玉米/花生间作选择塑造了二者的根际细菌群落结构,增加了特定潜在有益菌群的丰度,改善了根际微生态环境与功能,进而促进间作下产量优势的形成。

    Abstract:

    Cereal-legume intercropping pattern can promote the growth and yield of intercrops. Rhizosphere microbiome is believed to be closely associated with yield advantages of intercropping systems. Therefore, the pot experiments were conducted in this study for maize-peanut intercropping with three different root barrier treatments including plastic-film separation (namely complete separation, denoted as CM for maize and CP for peanut), nylon-net (50 μm) separation (denoted as SM for maize and SP for peanut) and no separation (denoted as NM for maize and NP for peanut). High-throughput 16S rRNA sequencing and PICRUSt functional prediction were applied to analyze the changes in bacterial community structure and functional categories in both maize and peanut rhizosphere under different intercropping patterns, in order to explore the ecological mechanisms of yield advantages of maize-peanut intercropping from the perspective of soil microbiome. The results showed that maize-peanut intercropping remarkably affected their bacterial community structure in rhizosphere. The bacterial communities in NM and SM were more similar and were considerably different from CM. However, the bacterial communities in SP were distinctly different from NP and CP. Furthermore, it was found that the relative abundance of Actinobacteria was significantly higher in NM and SM than in CM, and the relative abundance of Actinobacteria and Firmicutes were significantly higher in SP than in NP and CP. By contrast, the relative abundances of Gemmatimonadetes showed the opposite trend in both maize and peanut rhizosphere. These operational taxonomic units (OTUs), which were more abundant in nylon-net separation and no separation than in complete separation in both maize and peanut rhizosphere, were mainly assigned to the phylum Actinobacteria. Co-occurrence network analysis indicated that Streptomyces, Actinomadura, Arthrobacter, and Bacillus were the keystone nodes in maize bacterial network, showing a significantly higher abundance in NM and SM than in CM. Similarly, the keystone nodes in peanut bacterial network including Streptomyces, Actinomadura and Arthrobacter showed a significantly higher abundance in SP than in NP and CP. Lastly, PICRUSt-based functional prediction showed that maize-peanut intercropping significantly increased the relative abundances of functional categories including membrane transport and carbohydrate metabolism but decreased the abundance of the functional category related to replication and repair. In conclusion, maize-peanut intercropping selectively shaped the bacterial communities in both maize and peanut rhizosphere with an increase in the relative abundances of several potential probiotics, which improved the rhizospheric micro-ecological environment and functions and thus promoted the formation of yield advantages of intercropping.

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吴林坤,李奇松,李倩,方媛,蔡思思,林文雄.玉米和花生间作根际细菌群落结构与PICRUSt的功能预测.生态学报,2023,43(18):7485~7496

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