Grasslands are important contributors of carbon sequestration among terrestrial ecosystems, which play an important role in the global biogeochemical cycle and energy exchange. Based on field surveys in July-August 2021, soil samples of meadow steppe, typical steppe and desert steppe in Ningxia were collected. The organic carbon contents, bulk density, total nitrogen, and pH of these soil samples were measured. We also monitored factors affecting carbon distribution such as altitude, annual precipitation, annual mean temperature, aboveground biomass and soil water content. Results indicated that content of the soil organic carbon decreased along the vertical soil profile, the average organic carbon content followed the order of meadow steppe > typical steppe > desert steppe. The distribution of grassland soil organic carbon gradually decreased from the Liupan Mountains in the south to the dry sand belt in the middle along the altitude. Soil organic carbon was significantly positively correlated with aboveground biomass, annual precipitation, altitude and soil total nitrogen density, and significantly negatively correlated with annual mean temperature (P<0.001), positively correlated with soil water content, and negatively with soil bulk density (P<0.01), and no obvious correlation with soil pH. Amos structural equation model was applied to evaluate the combined effects of environmental factors on grassland soil organic carbon. The most important factors affecting organic carbon was altitude (r=0.78), which mainly caused regional differentiation of precipitation and temperature to affect soil organic carbon indirectly; followed by aboveground biomass (r=0.559); then annual precipitation (0.539), which not only directly but also indirectly affected the soil organic carbon by altering aboveground biomass and soil water content positively. Annual mean temperature indirectly affected organic carbon negatively by influencing aboveground biomass (r=-0.259). The spatial distribution of grassland organic carbon in Ningxia was dominated by precipitation and aboveground biomass closely related to precipitation. In the past 20 years, with the increase of precipitation and the reduction of grassland anthropogenic disturbance, grassland ecosystem in Ningxia had a very significant carbon sink function.