Coordination of ecological network pattern and urban network pattern is an important topic of urban sustainable development. Based on the land use data of Chengdu-Chongqing urban agglomeration in 2000, 2010 and 2020, we applied MSPA analysis, landscape pattern index, landscape connectivity and MCR model, used ecological sources and ecological corridors, economic and social sources and traffic corridors to construct the regional ecological network pattern and urban development network pattern in the study area. This study analyzed characteristics and evolution trends of four types of spatial elements (point, line, plane and network) formed by node, corridor, source and network, and discussed the spatial relationship and spatio-temporal changes of the regional dual network pattern. The results showed that:(1) from 2000 to 2020, the total area of the ecological sources and the ecological corridors in the study area was affected by urbanization to different degrees, and the total area decreased by 2660.26 km² and 1169.9 km² respectively, while the total area of the economic and social sources and the traffic corridors increased by 4324.19 km² and 2392.14 km², respectively. (2) from 2000 to 2020, the center of gravity of the ecological key points in the high density area with central Chongqing and southwest Sichuan as the core moved from east to southwest, and then moved back to east and northeast, and the number of ecological key points decreased by 311. Meanwhile, the number of ecological disturbance points in 2020 increased by three times than that in 2000. (3) from 2000 to 2020, the disturbance of traffic corridors to ecological sources increased, and the percentage of intersecting patches and length of intersecting corridors between them increased by 13.13% and 33.08%, respectively. According to the analysis results, four strategies, including node response, corridor optimization, source protection and network connection, are proposed to provide the basis and reference for the protection of the national ecological space and regional new-type urbanization.