In the context of global climate change, the increasing surface ozone concentration in China has seriously threatened the forest ecosystem. However, the research on the effects of ozone pollution on the biomass of subtropical forests in China is still uncertain. In this study, we compared the difference of biomass loss rate between forest and understory grassland assessed by different models and different parameterization schemes, and compared the consistency of biomass loss rate and total primary productivity (GPP) loss rate between broad-leaved forest and coniferous forest and understory grassland in Dinghushan. The biomass loss rates of broad-leaved forest, coniferous forest and grassland were 11.3% to 11.69%, 3.97% to 3.68% and 11.2% to 14.6%, respectively, caused by ozone pollution in Dinghushan during 2015-2016. The biomass loss rate of broad-leaved forest, coniferous forest and grassland varied from 9% to 13%, 3.68% to 4.4% and 11.2% to 14.6%, respectively. The GPP loss rate of broad-leaved forest, coniferous forest and grassland was estimated to be 10% to 12.6%, 1.81% to 2.6% and 3.2% to 3.3% based on ozonometry response model. In general, the biomass and GPP loss of broad-leaved forest and coniferous forest in Dinghushan had a high consistency, and the loss rate of broad-leaved forest biomass and GPP was significantly higher than that of coniferous forest.