Making full use of rural cultural resources, and realizing the values of rural cultural ecosystem services (CES) are important ways to meet the demands of urban and rural residents, develop a new economic growing field, and promote cultural revitalization in rural areas. Taking the rural areas of Huzhou City, China as an example, this study adopted geographic data, social survey data, and Points of Interest to optimize the indicator assessment system of the Maxent model, and map the cultural ecosystem services. The research also depicted the characteristics of the distribution of cultural ecosystem service values in the rural areas of Huzhou City, by analyzing the spatial autocorrelation of the supply and demand of the cultural ecosystem services. The study discovered that:(1) Among the three types of cultural ecosystem services in rural areas of Huzhou City, the value of scientific and educational services was the highest, followed by the values of aesthetic services and recreational services. The recreational services covered the largest areas of the research areas, followed by the aesthetic services, and then the scientific and educational services. In terms of spatial distribution, the north of Deqing County, the south of Anji County, and the northwest of Changxing County were the areas with high cultural service value. (2) The most important factors contributing to the Maxent model analysis were the Points of Interest, the land use and land cover, and the distance to the road. With the assistance of Points of Interest, the research enhanced the reliability of results, as well as the mapping accuracy of cultural service value. (3) Combined with the resident population and tourist population, most areas of Wuxing district had the highest demand for cultural services, and the rest were the north and middle of Anji County, Changxing County, the east of Deqing County, and Nanxun district. (4) The rural areas of Huzhou City were categorized into four categories based on the matching relationship between the supply and demand of the cultural ecosystem services. They were developed areas, demand areas, potential areas, and development areas. The geographical areas covered by the four cultural ecosystem services were similar, but the distributions of these four kinds of areas were highly heterogeneous. The purpose of this study was to explore a research method for the development path of rural cultural ecosystem services based on the perspective of supply and demand, and to provide theoretical support and suggestions for the comprehensive development of rural cultural ecosystem services.