The construction of ecological network is an important measure to connect habitat patches and protect biological habitats, which not only can improve ecological function, but also increase the comprehensive ability of the ecosystem and alleviate the deterioration of ecological environmental. It is of great significance to guarantee urban and regional ecological security and improve human well-being. Taking the urban agglomeration of Guangdong-Hong Kong-Macao Greater Bay Area as an example and relying on the scene planning method, this study carried out the scene elements and collaborative path analysis of ecological network construction. Based on the Morphological Spatial Pattern Analysis (MSPA) and the Minimum Cumulative Resistance model (MCR), we identified the ecological sources, ecological corridors and ecological nodes to form the ecological network of the Greater Bay Area. In addition, the potential collaborative forms of urban agglomeration ecological network construction were discussed from a cross-regional perspective. The research results show that:(1) ecological network of the Greater Bay Area is composed of 40 ecological sources, 780 ecological corridors and 892 ecological nodes, which has a high overlap with nature reserves, ecological belt in Guangdong and greenway network in the Pearl River Delta. The ecological source is 18800 km², accounting for 33% of the Greater Bay Area. (2) 552 cross-regional ecological corridors are identified and more than half of them across three or more cities. The corridors crossing Guangzhou and Foshan are as high as 46%. The land-sea ecological corridor connects the major coastal cities in the southern part of the Greater Bay Area, forming a key component of the land-sea ecological network. (3) A potential collaborative mode for ecological network construction of urban agglomerations was proposed. From the perspective of ecological source, ecological corridor and ecological node, collaborative needs, collaborative objects, and collaborative paths were further analyzed from the intra-city and inter-city scales. (4) Scene planning helps to identify the scene theme, spatiotemporal characteristics, scene objects, scene construction paths and scene value of urban agglomeration ecological network construction. It can be a theoretical tool to provide guidance for the further development of the ecological network collaborative construction and practice. This research is a useful exploration of the collaborative construction of ecological network in urban agglomerations. The discussion based on the collaborative construction of cross-regional ecological corridors will helps to provide a theoretical basis for further exploring regional coordination mechanisms, realizing the collaborative construction and optimization of landscape patterns, and promoting regional ecological joint construction in the future.