Huzhou is the origin of the Beautiful Countryside Construction in China. The rural landscape has changed greatly in the past decade, which has made pressure on habitat maintenance and cultural protection. Assessing ecosystem services and their relationships is important for the sustainable development of rural areas of Huzhou. Based on Thornthwaite Memorial model, Miami model, InVEST model, Water Balance method, Maxent model, and others, this study quantitatively evaluated the ecosystem services of food supply, carbon storage, water conservation, and cultural ecosystem service in rural areas of Huzhou from 2010 to 2018, and then analyzed the trade-offs and synergies among different ecosystem services. The results showed that (1) the food supply of rural areas of Huzhou decreased in the fluctuation from 2010 to 2018. The first level regions were located in the east and west of the region, where were the cultivated land areas. The annual variation of carbon storage was small, and the high-value regions of carbon storage were located in the middle and west of the region, where were forest areas. The average value of water conservation decreased after a slight increase, and its spatial distribution characteristics were similar to that of carbon storage. The cultural ecosystem service increased, and the first level regions gathered around the scenic spots and expanded. (2) When analyzing trade-offs and synergies, the synergistic effects were dominant among water conservation, carbon storage, and food supply. In contrast, trade-off relationships existed between the cultural ecosystem service and other three services. The relationship between food supply and carbon storage changed from trade-off to synergy. The overall correlations among various ecosystem services were continuously enhanced and showed spatial differences. (3) The results of spatial heat map showed that, from 2010 to 2018, the proportion of hotspot I was large and increased slightly. The cultivated land was the main land type of hotspot I, whose dominant ecosystem service was food supply. The proportion of hotspot II has declined, distributing in forest areas. The ecosystem services of the hotspot II were carbon storage and water conservation. The hotspot III, including carbon storage, water conservation, and food supply or cultural ecosystem service, distributed in the cultivated land areas and forest areas, covered large areas and showed an increasing trend. Besides, the proportion of hotspot IV and non-hotspot covered small areas and changed little, located in the mountain-plain transition zone and near the urban areas, respectively. The results were expected to provide reference for revealing the relationships among ecosystem services in rural areas, optimizing the rural economic structure and development mode, and promoting rational planning and sustainable development for rural areas.