Abstract:The acorn barnacle Megabalanus volcano is a dominant fouling organism of coastal ecosystem, and inhabits at low intertidal and subtidal areas in tropical and subtropical shores. To explore the suitable culture conditions of M. volcano larvae, the effects of diets (species and density), salinity (18, 22, 26, 30, 34, 38, 42), and temperature (14℃, 18℃, 22℃, 26℃, 30℃, 34℃) on the larval development of M. volcano were evaluated. The survival percentage of naupliar Ⅵ, survival percentage of cyprids and development time of naupliar Ⅵ, development time of cyprids cyprid length were investigated as observation indicators. Skeletonema costatum (5×104/mL, 10×104/mL, 20×104/mL, 40×104/mL, 80×104/mL), Chaetoceros muelleri (5×104/mL, 10×104/mL, 20×104/mL, 40×104/m, 80×104/mL) and Platymonas subcordiformis (2.5×104/mL, 5×104/mL, 10×104/mL, 15×104/mL, 20×104/mL) were chosen as the diets of M. volcano. Results showed the following. Nauplii could not develop to cyprids at a low algal density for 3 algae, and could develop to cyprids over than a certain density. The larval development time decreased with algal density, however the larval survival percentage and the cyprid length generally increased with algal density. The highest survival percentage of cyprids on a diet of S. costatum (at 80×104/mL) was 41.33%, and the cyprid length was 681.43 μm. The highest survival percentage of cyprids on a diet of C. muelleri (at 80×104/mL) was 43.67%, and the cyprid length was 668.39 μm. S. costatum and C. muelleri were the suitable algae for rearing M. volcano. The optimum density for larval development on S. costatum and C. muelleri was 40×104/mL and 80×104/mL. The highest survival percentage of cyprids on a diet of P. subcordiformis (at 20×104/mL) was 14.33%,and the cyprid length was 554.60 μm. The survival percentage and length of cyprids on a diet of P. subcordiformis were much smaller than those on a diet of S. costatum or C. muelleri. Therefore, P. subcordiformis wasn't a suitable alga for rearing M. volcano. Nauplii of M. volcano could develop successfully to cyprids at a salinity range of 22-42 except at 18 salinity, the highest survival percentage of cyprids was 35.67% at 30 salinity, the larval development time was not significantly different among salinity groups, the cyprid length at low salinity (22 and 26) was longer than that at high salinity (30-42). The optimum salinity range for larval development was 26-34 salinity. Nauplii of M. volcano could develop successfully to cyprids at a temperature range of 18-30℃ except at 14℃ and 34℃, the highest survival percentage of cyprids was 50.67% at 22℃, the larval development time was significantly reduced as temperature increased; the cyprid length at low temperature (18℃) was longer than that at high temperature (22℃, 26℃, 30℃). The optimum temperature range for larval development was 22-26℃.In short, under the suitable alga, salinity and temperature conditions, larvae of M. volcano could generally develop to cypris phase after 8 days of rearing and the survival percentage of cyprids was up over 35%.