Based on the Normalized Difference Vegetation Index (NDVI) data of Moderate Resolution Imaging Spectroradiometer (MODIS) image and the relative meteorological data from 2000 to 2020, the temporal and spatial variation characteristics of NDVI in the growing season of Daqing Mountain National Nature Reserve in Inner Mongolia are analyzed from the perspective of elevation, slope and slope aspect by using the methods of trend analysis, coefficient of variation and correlation analysis. The correlation between temporal and spatial variation of NDVI and climate factors was further explored. The results show that:(1) The overall vegetation coverage of Daqing Mountain National Nature Reserve was good and growing from 2000 to 2020, with a growth rate of 0.058/10a. (2) The spatial distribution of the NDVI shows the characteristics of low in the west and high in the east, and gradually increasing from west to east of the reserve. With the increase of elevation, the mean value of NDVI in the growing season of the reserve increased significantly, but increased slowly with the increase of the slope. In different slope aspects, the mean value of NDVI in growing season was shady slope > semi-shady slope > semi-sunny slope > sunny slope. (3) From the analysis of NDVI change trend in 2000-2020, the area of vegetation coverage improvement in Daqing Mountain National Nature Reserve accounted for 61.75% of the total area, of which the areas of significant improvement and extremely significant improvement accounted for 41.13% and 20.62% of the total area, respectively, the area basically unchanged accounted for 38.17% of the total area, and the degraded area accounted for 0.07% of the total area. In terms of spatial distribution, the areas with the significant and extremely significant increase in NDVI were mainly distributed in the west and east of the reserve, and the change was not significant in the middle. According to the changing trend of NDVI in each functional area of the reserve, the NDVI increased significantly, and the area percentage increased significantly in the order of experimental zone > core zone > buffer zone. (4) From 2000 to 2020, the overall performance of stability is characterized by the coexistence of different fluctuations, the majority of low, medium and high fluctuations, and the proportion of low and high fluctuations being relatively small. The high fluctuations are mainly distributed in the western and central areas of the reserve and the experimental zone, and human disturbance and precipitation are the main driving factors. (5) The average annual maximum NDVI in the growing season of Daqing Mountain National Nature Reserve is mainly positively correlated with the average annual precipitation, and the proportion of the positive correlation area reaches 99.79%. It is mainly insignificant with temperature, and the insignificant area accounts for 97.91% of the total area. Precipitation is the main meteorological factor affecting the vegetation coverage of Daqing Mountain National Nature Reserve.