he relationship between the ecological stoichiometry of natural secondary forests and carbon and nitrogen isotopic abundance was analyzed to clarify the influence of element balance on isotope fractionation, as well as the ecosystem resource allocation mechanism and utilization strategy. The Pinus massoniana forest, Lindera glauca forest, Viburnum utile forest, Platycarya strobilacea forest, and Quercus fabri forest of dolomite district in Guizhou Province were used as the research objects to clarify the leaf-litter-soil C:N:P stoichiometry, carbon and nitrogen isotopic abundance and their intrinsic relationship. The results showed that:(1) there is a difference between carbon, nitrogen, and phosphorus contents in leaves, litter, and soil of different communities. The overall order is leaf> litter> soil. The inheritance of nitrogen in the continuum is strong, while carbon is weak. (2) The differences between C:N, C:P, and N:P ratios among the continuum of different plant communities are significant. The five communities are all restricted by nitrogen, while the Platycarya strobilacea forest and Quercus fabri forest are also restricted by phosphorus. (3) The δ¹³C values of leaves, litter, and soil are -32.45‰—-29.22‰, -30.11‰—-28.85‰, -26.06‰—-6.83‰, and the δ¹⁵N values are -8.36‰—-1.17‰, -6.79‰—-2.22‰, 3.22‰-7.12‰, respectively. Generally, there is consistency in the migration and transformation of elements. (4) In the leaf-litter-soil continuum, the correlation between stoichiometry and δ¹⁵N is higher than that of δ¹³C. Soil stoichiometry has a strong coupling effect with δ¹³C and δ¹⁵N. The results can provide theoretical references for the scientific management of ecosystem nutrients in dolomite district in Guizhou Province.