Catch and release is very popular as fish conservation strategy and fisheries management. The individuals who experience catch and release will have substantial changes in their physiology and behavior. When these individuals are faced with artificial bait again, they may show more cautious bait-taking or hook avoidance behavior. In order to investigate the effects of the repeated angling on vulnerability to angling, energy metabolism and personality of the Cyprinidae, juvenile crucian carp (Carassius auratus) was used for the animal model in our study. The morphology, metabolic rate (e.g., standard metabolic rate, SMR; the maximum metabolic rate, MMR) and personality (e.g., activity and boldness) of both the angling and control group were measured three times at (25.3±0.1) ℃. The individuals of the angling group were subjected to an angling experiment at each trial while fish in the control group did not experience any angling operation. The results show that the angling rate decreased significantly and the average individual angling time increased significantly as the increased of angling times. Repeated angling resulted in a higher death rate in the angling group compared to the control group. The body mass, condition factor, SMR, MMR, aerobic scope, and factorial aerobic scope were significantly decreased by the repeated angling in the angling group compared to the control group, but there were no changes in boldness and activity after the repeated angling. During the first test, high vulnerability to angling phenotype (HVA) had larger body length and movement time in open area, lower condition factor compared to low vulnerability to angling phenotype (LVA). However, during the second and third test, no difference in morphology parameters, energy metabolism, and personality (e.g., boldness, activity) could be detected between the HVA and LVA. Our study suggests that the repeated angling reduces the vulnerability to angling in juvenile crucian carp, and the change direction in vulnerability to angling are different among groupmates with the angling group. Repeated angling decreases aerobic scope of the juvenile crucian fish, which may reduce potential metabolic scope for physiological functions such as exercise, growth and digestion, and ultimately reduce the survival fitness of the individual.