The construction of ecological network is particularly important for ensuring regionally ecological security, especially for improving landscape connectivity and protecting biodiversity. It is also a crucial content of ecological restoration of the territorial space. Currently, the basic pattern of ecological network construction based on landscape ecology has been formed. However, the methods of three core steps under this pattern, including "identification of ecological source, construction of comprehensive resistance surface, and generation of ecological network", are diversified. Considering the scale effect and the spatial heterogeneity of landscape, it is necessary to explore the applicability of different approaches through comparative evaluation. In this study, four methods of ecological source identification, two methods of comprehensive resistance surface construction and two methods of ecological network generation were selected as the research methods. Based on the two perspectives of the minimum cumulative resistance model and circuit theory, the corresponding ecological network was formed under the combination of the above methods for comparative analysis. The results showed that:(1) due to the difference of principles, the analysis results of different methods in each step were significantly different, which were mainly reflected in the number, area and spatial distribution of ecological source areas, the distribution of high resistance values on the resistance surface, and the number and pattern characteristics of ecological corridors and ecological nodes. (2) In the comparison scheme 1 based on the principle of the least cumulative resistance model, an evaluation system is constructed including Harary index, overall connectivity index IIC, possible connectivity index PC, cost ratio λ, network density D, efficiency robustness E and corridor density T. According to the comprehensive evaluation results, the optimal ecological network was constructed by the combination mode of ecological source identification based on comprehensive evaluation method, construction of comprehensive resistance surface based on resistance coefficient combined with night light correction factor and generation of ecological network by Graphab software. (3) In the second comparative scheme, the method based on the circuit theory could identify potentially ecological corridors and generate the results used to replace the minimum-cost-path type of ecological corridors, providing a good complement to the minimum cumulative resistance model. Therefore, combining the two methods can effectively improve the level of ecological network security.