To understand the spatial and temporal variation of fish assemblage structure and biodiversity, as well the relationships between fish taxonomical diversity and functional diversity, species compositions, the dominant species, taxonomical and functional diversity of fish communities which were captured from Zhelin Reservoir from September 2020 to April 2021 were investigated and analyzed. The results showed that a total of 53 fish species belonging to 5 orders, 12 families, and 36 genera were sampled with dominant Cyprinidae accounting for 71.69% of the total species. Among the reservoir spatial scales, 36, 40 and 32 fish species which accounting for 72.97%, 85.00% and 75.00% of the total sampled species were captured from upper-, middle- and low-reach, respectively. Hemiculter leucisculus, Aristichthys Nobilis and Xenocypris davidi were dominant species, while Ctenopharyngodon idella, Hypophthalmichthys molitrix, Parabramis pekinensis, Carassius auratus and Erythroculter mongolicus were main species in Zhelin Reservoir. The analysis of PERMANOVA (Permutational Multivariate Analysis of Variance) using the fish assemblage matrixes showed that the seasonal variation of species compositions was more significant than the spatial scale, and the variations were mainly performed between two seasons of antum and summer. Meanwhile, biodiversity indexes and functional diversity indexes of fish assembalges performed relative higher variations among spatial scales than temporal scales. Margalef index were relatively higher in the Xiuhe River than those in other water sites, while the indexes of Shannon, Simpson and Peilou indexes showed no variation among the reaches. Functional richness possessed relative higher level in upper reach than midlle and lower reaches, while functional evenness and functional disperse performed non changes among spatial scales. Functional redundancy had no variation among spatial and temporal scales. Regression analyses showed significant positive relationships between the biodiversity index of functional richenss and functional disperse with each taxonomical diversity index, while there were negative relationships between functional redundancy and taxonomical diversity indexes, which implying the increase of species richness led to the increase of the functional richness and decrease of functional redundancy. However, functional evenness did not change obviously which can mainly be explained by the disproportionate individuals of different fish functional groups. The results implied that interference resulted in a lower niche overlap of fish community with high species richness and lower ecosystem recovery ability. In general, the species diversity level of Zhelin Reservoir is lower and the anti-interference ability of this ecosystem is relatively weaker. To protect fish biodiversity and maintain the stability of the ecosystem, it is necessary to improve the fish community redundancy and the availability of resources and habitats by regulating the fish population structure.