Cultural Ecosystem Services (CES) are the non-material benefits people obtain from nature. They play a key role in improving human well-being and maintaining environmental sustainability. The core of CES is the emotional connection between human and nature. Conducting participatory mapping from the perspective of stakeholders is an effective way to perceive and protect hot CES spots. A participatory mapping and interview questionnaires were used to investigate the perception of CES between tourists and residents in Wuyishan City, which is a county-level city under the jurisdiction of Fujian Province of China. It is the main location of Mount Wuyi, which is a UNESCO World Cultural and Natural Heritage Site and Wuyishan National Park. We compared the influencing factors and spatial pattern distribution of tourists' and residents' perception difference of CES in our study area, using spatial analysis and hotspot analysis methods. The results showed that:(1) except that residents' monthly income had an impact on their perception, the perception of CES of tourists and residents was basically not affected by demographic characteristics (gender, age, occupation and education level) (P>0.05). (2) The residents' per capita willingness to pay for protection in CES value was higher than tourists. Then, cultural heritage, recreation and ecotourism, education, and aesthetic value were four sub-cultural services valued by both tourists and residents. Tourists had the highest willingness to pay for cultural heritage value services, while residents had the highest willingness to pay for educational value services. (3) Residents' perception of CES value existed in the spatial distribution of a wide range, almost covering most towns and villages in Wuyishan City, while tourists were mainly located in the area with dense tourist destinations. The degree of perception of CES value on cold- or hot-spots had similar spatial distribution patterns to tourist spots. The intensity of perception of CES value from both tourists and residents decreased gradually from south to north of Wuyishan City. (4) There was no correlation between spiritual and religious values and recreational and ecotourism values, while there was a high correlation among the other sub-cultural services. For tourists, aesthetic value had the strongest correlation with inspiration value, while aesthetic value had the strongest correlation with local identity value among residents. Therefore, it is of great significance to identify and consider the perception differences between residents and tourists in CES to improve the regional planning level and landscape service functions. Integration the link between CES and civil society's engagement in environmental stewardship is beneficial to achieve more harmonious relationship between man and nature in our study area.