Abstract:Every January from 2017 to 2020, the investigation of the hibernating ecological characteristics of bats in tunnels of the artificial canal of Budaigou Reservoir in Shaoyuan Town, Jiyuan City, Henan Province, was operated. Seven bat species, which belong to 5 genera in 2 families, were found hibernating in the tunnels, including Rhinolophus ferrumequinum, R. pusillus, Myotis laniger, Murina leucogaster, Mu. aurata, Plecotus ognevi, and Barbastella leucomelas. The dominant species was R. ferrumequinum (about 52%-73% hibernators), followed by R. pusillus (about 19%-37%), and M. laniger (about 5%-8%). While other bat species were less than 3%. The total number of hibernating bats showed an increasing trend over years, but it was still less than that reported earlier. And there were 42 tunnels with bats hibernating in every year, and the number of hibernators in each tunnel varied annually. The tunnel length was the major effector on the selection of hibernacula, based on the multiple linear regression analysis (adjusted R2=0.208, P=0.001). The study revealed that bats had different hibernating roost-points in each tunnel, and about 4/5 bats hibernated in the deeper section of tunnels (> 30 m), where the environment was warmer and relatively stable, which was affirmed that more than 95% of individuals hibernated in tunnels with length > 60 m. Bats had different hibernating patterns, for example, most individuals hibernated in solitary (>90%), while the others aggregated in clusters. The different hibernating roost-points and patterns might facilitate the optimization of hibernation cost. In addition, there was a significant positive correlation between roost-point temperatures and body temperatures (R2=0.98, P<0.001), and the roost-point temperatures required for hibernation had intraspecific and interspecific differences. The above results provided a scientific basis for the protection of bat populations and management of hibernacula in China.