Soil CO₂ is a key driving force for karst processes, as well as the link between carbon cycle in terrestrial ecosystem and karst carbon cycle. A typical soil profile of meadow ecosystem in karst region of Slovenia was selected for the purpose of implementing high resolution monitoring of parameters, including contents of atmospheric CO₂ and soil CO₂, soil temperature and soil moisture, etc. The variations with days and at different depths of these parameters were analyzed, and the layered effect and parameters' relationship also were discussed. The results showed that soil temperature, soil moisture and soil CO₂ content ranged from 3.8℃ to 12.9℃, 26.9% to 34.7% and 682×10^-6 to 6760×10^-6 respectively. Soil temperature changed in accordance with air temperature; moreover, soil temperature at upper layer was much sensitive to air temperature thus presenting daily variation, while lower layer showed less daily fluctuation. Soil moisture mainly was controlled by rainfall and responded to rain events promptly. Layered effects at different depths were remarkable both in soil moisture and soil CO₂. Soil moisture lowered and then raised with increase of soil depth, implying that grass roots were favored for soil moisture maintenance, and high moisture content at lower soil part might be caused by the halt of rock-soil interface below. Soil CO₂ content was overall controlled by soil temperature, presenting a significant positive correlation each other. During the rain events, soil CO₂ contents at various layers all exhibited behavior of "rapid ascending and descending", suggesting that it was possibly resulted from the pulse effect caused by rainfall infiltration and consequent downward movement. Additionally, atmospheric CO₂ content also showed a remarkable decrease during the rainfall, which could be explained that CO₂ both from atmosphere and soil were involved in underlying carbonate rock dissolution. Rainfall processes can not only mitigate soil CO₂ emission to atmosphere, but also dissolve atmospheric CO₂ and flow into the underlying karst aquifer system. This observation revealed that ecosystem controlled CO₂ acted as an important driving force for underlying carbonate rock dissolution in wet/cold Mediterranean climatic karst region. The resulting carbon sink could be an important part of the whole ecosystem carbon sink. Accordingly, it is suggested that carbon sink enhancement resulted from karst processes should be considered as well as carbon sink increase from vegetation restoration and soil amelioration in the development of carbon cycle monitoring plans and carbon sink estimation in karst area, especially in implementation of comprehensive treatment projects of rock desertification.