The concept of biotope is widely used in ecological and biological protection. By expounding the definition, connotation and extension of the wetland biotope, this paper analyzed characteristics of the wetland biotope including hierarchy, diversity, and obviously ecological differentiation. We introduced some application cases of the internal biotope of the wetland ecosystem, the wetland "foreign aid" biotope and the artificial wetland biotope in wetland restoration. In addition, the authors summarized the research directions (such as coupling relationship between wetland biotope and biodiversity) which the wetland biotope needs to pay attention to in the future, in order to provide a theoretical and scientific basis for wetland protection and fine management.