Jiuzhaigou County is an attractive tourism county in China, and the regionally social and economic development is closely related to the ecosystem service values (ESVs) of Jiuzhaigou National Nature Reserve. In 2017, a 7.0-magnitude earthquake occurred in Jiuzhaigou National Nature Reserve, which severely damaged the local ecological environment and regional social and economic development. Based on the ecologic environment status of Jiuzhaigou, taking 2016 and 2018 as the base years of before and after the earthquake respectively, seven indexes were selected from three aspects of provisioning services, regulating and maintenance services and cultural services to construct the ESVs evaluation system of Jiuzhaigou National Nature Reserve and identify the change of ESVs before and after earthquake. The results show that (1) the total ESVs of Jiuzhaigou before and after the earthquake were estimated to be 1.96×10¹⁰ yuan/a and 1.52×10¹⁰ yuan/a, respectively, while the unit area ESVs were 3.05×10⁵ yuan hm^-2 a^-1 and 2.36×10⁵ yuan hm^-2 a^-1, with a decrease of 22.68%. (2) The three types of ecosystem service values all decreased before and after the earthquake, but the sequence of the ESVs proportion unchanged, followed by provisioning services, regulating and maintenance services and cultural services, with a decline of 24.61%, 12.25% and 86.02%, respectively. (3) Before the earthquake, the ESVs were in the order of wood supply, water retention, soil conservation, forest recreation, carbon fixation and oxygen release, existent of Giant Panda (Ailuropoda melanoleuca) and scientific education, and the top three services accounted for 92.80% of the total values. After the earthquake, the order of the ESVs changed into wood supply, water retention, soil conservation, carbon fixation and oxygen release, existent of Giant Panda (Ailuropoda melanoleuca), scientific education and forest recreation, while the top three ESVs accounted for 96.93% of the total values. (4) Among the three types of ecosystem services, cultural services were greatly affected by the earthquake, with the proportion of ESVs decreased from 4.54% to 0.82%. Among the seven indexes of ecosystem services, only the values of scientific education and soil conservation increased, while the values of the other five ecosystem services all decreased to various degrees, and the decreasing order was forest recreation, carbon fixation and oxygen release, wood supply, existent of Giant Panda (Ailuropoda melanoleuca) and water retention. Due to the closure of Jiuzhaigou National Nature Reserve, the values of forest recreation measured by tourism revenue changed the most, with a decrease of 97.74%, indicating that the geological disasters caused a cliff-like decline in tourism industry and had a huge impact on the development of social economic in Jiuzhaigou County.