Based on remote sensing and field survey data, the spatial distribution pattern and influencing factors of amphibians and reptiles in Beijing were studied. The results showed that 22 species of amphibians and reptiles were recorded, belonging to 17 genera, 10 families, 3 orders, 2 classes. Among them, there were 15 protected species, accounting for 68.19% of the total species. The spatial distribution of amphibians and reptiles in Beijing decreased from northwest to southeast, and the Northwest Mountainous Areas had higher species richness and relative abundance. The highest species richness (14 species) was in Mentougou District and Yanqing District, accounting for 66.67% of the total species, in which the dominant species were R. chensinensis, P. nigromaculatus, B. gargarizans, and E. brenchleyi. The population in Mentougou, Huairou and Fangshan accounted of 71.54% of Beijing. The highest richness (18 species) was at the altitude 200-500 m, and which main habitat was forest shrub. There were 11 grids with more than 6 species, including Datan, Huaijiu Lake, Baihua Mountain, Juma Lake, Miyun Reservoir, and so on. In general, the species richness and its population number showed a decreasing trend, and the most important impact was land use conversion. During 1980-2018, the construction land presented the trend of expanding to the periphery with the core of Dongcheng District and Xicheng District. The occupied farmland accounts for 42.85% of the total conversion area of the ecosystem. The main species in the six urban districts and plain area are Pelophylax nigromaculatus and Bufo gargarizans, and Rana chensinensis is few. Within the 1500 m migration distance of amphibians, the main habitat types are forest, shrub and farmland, and some farmland is occupied by urban expansion. Pollutants caused the decline of water quality resulted in habitat destruction and the decline of amphibian breeding rate. All kinds of protected areas in Beijing cover 90.91% of the habitat and 60.45% of population of amphibians and reptiles, but there are still some protection gaps in the upper section of Baihe River in Baoshan Town of Huairou District, Xinzhuangqiao in the upper section of Chaohe River from Miyun Reservoir, Daxingzhuang to yukou town of Ruhe River in Pinggu District. Therefore, it is suggested to carry out long-term positioning observation, build Biodiversity Observation Network, and explore the establishment of biodiversity protection mechanism under high-intensity land use, so as to realize the protection and sustainable utilization of biological resources.