With the rapid development of social economy, land use types change dramatically which has an important impact on the ecosystem service value. Therefore, quantitatively estimating the impact of land use change on the ecosystem service value is of great significance to restore and construct regionally ecological environment. Based on the ecosystem service value accounting system and the land use data of Dianchi Lake Basin in 2010 and 2016, the influence of land use change and its mutual transform on ecosystem service value was evaluated. The results show that, the annual ecosystem service value of Dianchi Lake Basin grew from 68.816 billion to 72.931 billion Yuan during the period of 2010 and 2016. The order of the ecosystem services value from different land use types was:forest land > water area > cultivated land > built-up land > grassland > bare land. The order of the land use change degree was:built-up land > cultivated land > grassland > forest land > bare land > water area. The structure of land use changed significantly from 2010 to 2016. The type with the greatest dynamic degree was built-up land which had been increased by 93.25 km². The following was cultivated land which had been decreased by 52.71 km². During the period of 2010 and 2016, the ecosystem services value of Dianchi Lake Basin due to mutual transition among land use was increased by 0.388 billion Yuan. The ecosystem services value increased by 5.462 billion Yuan because of the changes in cultivated land, grassland, built-up land, and bare land, and decreased by 5.074 billion Yuan because of the changes in forest land and wetland. This study can provide an important reference for the coordinated promotion of high-quality economic and social development and high-level eco-environmental protection in Dianchi Lake Basin.