Biogenic volatile organic compounds (BVOCs) are low-boiling, small-molecule secondary metabolites released by plants, and play an important role in the biosphere, such as herbivore deterrence, fungal toxicity, and pollinator attraction. Besides, BVOCs are also important gaseous compounds in the atmosphere, which can react with active free radicals in the atmosphere to form secondary organic aerosols (SOA) and prolongs the life of greenhouse gases such as CH4 in the atmosphere. Analyzing the impact of environmental factors on Biogenic Volatile Organic Compounds (BVOCs) emissions is of great significance for accurately understanding future climate change. As we all know, nitrogen is one of large numbers of nutrients for plant growth, however, it's increasing in deposition is the main driver of current global climate change. Besides, the emission of BVOCs in response to the elevated N deposition is poorly understood. In this study, Schima superba, Cryptocarya chinensis, and Syzygium araiocladum were chosen as the research objects, which are the tropical rain forest tree species in Hainan Island, the short-term effect of nitrogen deposition on the release of BVOCs of three tree species were also simulated through a greenhouse test. A dynamic headspace method was used to collect BVOCs released by the three broad-leaved tree species. The main conclusions are as follows:(1) Under natural conditions, 14, 34 and 24 kinds of volatile organic compounds were isolated and identified from the branches and leaves of S. superba, C. chinensis, and S. araiocladum, including isoprene, monoterpene, sesquiterpene and other VOCs (alkanes, carbonyls, aldehydes, alcohols, esters, ethers and acids), and the rates of BVOCs released by three broad-leaved tree species showed C. chinensis > S. superba > S. araiocladum.. (2) Nitrogen application promoted the release of BVOCs from the three plant seedlings, and the effect of foliar nitrogen application was significantly higher than that of soil nitrogen, and the emission rate of Total Volatile Organic Compounds (TVOCs) and the number of components increased with the increase of nitrogen concentration. (2) The sensitivity of the three plant seedlings to nitrogen deposition was expressed as Schima superba > Cryptocarya chinensis > Syzygium araiocladum. (3) Nitrogen application had an impact on plant physiological parameters and soil fertility, and the effect of foliar nitrogen application was more prominent. Among the physiological parameters, the net photosynthetic rate had the closest relationship with the BVOCs released by the seedlings, followed by the stomatal conductance. Nitrogen application mainly had a significant effect on soil pH, NH₄⁺-N and NO₃^--N, followed by Available phosphorous (AP) and Total phosphorus (TP).