Ecological space, the core area of land planning, is an important part of maintaining regional sustainable development. Ecological space zoning is a key content of land management. It is also an important means to solve the imbalance between regional supply and demand of ecosystem services and to maintain land security. This paper systematically summarized the existing methods of ecological zoning, discussed the shortcomings of the current ecological zoning, further put forward the theory and connotation of ecological zoning and the technical points of ecological space zoning under the land planning. Ecological space zoning is based on ecological space providing ecosystem services and maintaining ecological security. At the regional scale, it comprehensively identified and classified ecological space with mountains, rivers, forests, farmlands, lakes, and grasslands as a life living community. We put forward ecological space zoning based on the ecological function zoning and environmental function zoning, combined with policy goals, the needs of residents, the characteristics of regional urbanization, and future development. Multi-factor evaluation of the ecosystem services, ecological sensitivity, ecological quality, and ecological health should be taken into account. The purpose of ecological zoning is to protect, restore, and high-quality develop ecological space. Therefore, the threshold of ecological zoning should be based on the policy goals, the needs of residents, and sustainable development, according to the coupling mechanism of supply and demand of ecosystem services, and be adjusted in time. Based on the difficulties of ecological zoning at the present stage, this paper also put forward the future work of ecological zoning from the aspects of strengthening the application of ecological zoning system, strengthening the construction of ecological space basic database, and strengthening the naming standard.