Chenyao Lake basin is an important waterfowl Provincial Nature Reserve in the middle and lower reaches of the Yangtze River,In recent years, with the development of agriculture, there are some hidden dangers in the lake area, such as planting too much Euryale ferox, affecting the flood discharge in flood season, and seriously affecting the stability of the structure and function of the aquatic ecosystem in the lake area.In order to study the effects of mowing experiment in different areas of Euryale ferox on the community structure of metazoan zooplankton in Chenyao Lake, the metazoan zooplankton was investigated in August 2019. A total of 40 species of metazooplankton belonging to 22 genera, 15 families were identified. Among them, 26 species belong to 12 genera of Rotifer, 11 species belong to 8 genera of Cladocera and 3 species belong to 2 genera of Copepoda,The community structure was dominated by Rotifers,There was no significant difference in the spatial and temporal distribution of metazoan zooplankton (P>0.05). A total of 8 genera and 10 species of dominant species were found,including Brachionus calyciflorus, B. angularis, B. forficula,Keratella cochlearis, Monostyla bulla, Lecane ungulata, Polyarthra trigla, Filinia maior, Bosmina longirostris and Mesocyclops leuckarti. The average animal density and biomass of metazoan zooplankton were (1681.49 +205.73) ind/L and (7.8+1.52) mg/L respectively,The peak values of density and biomass appeared in the initial stage of no mowing area and the lowest values all appeared in the later stage of channel.The spatial distribution of the density of metazoan zooplankton showed the characteristics of no mowing area > mowing area > river channel, while the temporal change trend was in the initial stage > early stage > middle stage > late stage. The trend of spatial and temporal variation in biomass and density was consistent. The Shannon Wiener diversity index, Margalef richness index and Pielou evenness index of the metazoan zooplankton community were 1.67-3.42, 1.64-3.57 and 0.41-1.13, respectively. The results of Redundancy analysis and Pearson correlation analysis showed that TN, TP, NH₄⁺-N, Chl a, EC and SD were the main factors affecting the community structure of zooplankton. According to the comprehensive biological and physical and chemical indicators, Chenyao Lake was in a state of moderate pollution. The results of nutritional parameters indicated that Chenyao Lake was in a state of moderate eutrophication.