The systematic reconstruction of tourism rural ecological space is the key to the sustainable development of rural areas. Taking Wolong Village as a typical study area which located in Chaling County, this paper adopts participatory rural appraisal method to invert the different development stages of ecological space and analyze the evolution characteristics, and uses the quantitative methods such as ecological sensitivity evaluation and the least resistance model to systematically analyze the components of ecological space and explore the reconstruction methods of ecological space. It is found that the ecological space of Wolong Village is composed of 6 pieces of ecological source, 15 channels of ecological corridors, and 22 points of ecological nodes.The spatial distribution of various elements is unbalanced, the spatial structure is unreasonable, and the ecological function is not strong. According to the analysis of four stages of ecological space development, the evolution features of ecological space show obvious scale decline, spatio-temporal difference and functional complexity. In order to reconstruct the ecological space, new ecological sources should be added, ecological corridors and nodes should be adjusted or upgraded. In addition, a reconstruction path of 7 ecological sources, 14 ecological corridors and 19 ecological nodes should be constructed, and measures such as the classified control of spatial elements, optimization of ecologically spatial structure and guidance of ecological planning should be taken. The study of ecological space reconstruction provides theoretical reference and case reference for rural ecological protection and rural revitalization strategy implementation.