Abstract:As an important part of China, the Qinghai-Tibetan Plateau plays a vital role in the balance of global ecosystems. Climate change and human activities have led to grassland degradation on the Qinghai-Tibetan Plateau, especially overgrazing-induced grassland degradation. However, as one of the most dominated livestock on the Qinghai-Tibetan Plateau, the effects of different yak grazing intensities on soil physicochemical properties and microbial communities and structures still remain unclear. To address yak grazing effect on soil bacterial communities, we conducted a yak grazing intensity experiment in a typical alpine meadow of the Northwest Sichuan Plateau. According to local investigations and previous experiment, we set four grazing intensities, including control group (no grazing), light grazing (1 yak/hm2), moderate grazing (2 yak/hm2), and heavy grazing (3 yak/hm2). After two years of grazing experiments, we found that heavy grazing significantly increased soil available phosphorus and short term grazing reduced the bulk density in the 0-10cm soils, while other soil properties showed no responses to different grazing intensities. The possible reason may be that the effect of grazing on soil physicochemical properties was long-term, and the short-term grazing intensity of yak was difficult to change soil properties. The α diversity of soil bacteria increased firstly and then decreased with the increasing grazing intensity, but the changes were not statistically significant due to the short duration of grazing experiment. On the one hand, yak grazing could provide available nutrients for the growth of bacteria through dung and urine. On the other hand, high grazing intensity changed the root distribution and root exudates of plants, and increased ultraviolet radiation reduced the diversity of bacteria. In terms of the dominant bacteria, the Chloroflexi was significantly higher under no-grazing than that under heavy grazing, which has photosynthetic autotrophic bacteria. In contrast, other phylum had no significant differences among grazing intensities. With the increase of grazing intensity, the relative abundance of Actinobacteri and Firmicutes increased firstly and then decreased, while Acidobacteria and Gemmatimonadetes showed reverse trends. The β diversity of soil bacteria showed that grazing intensity had no significant effect on bacterial community structure. Soil total nitrogen is positively correlated with Nitrospira. There was a significantly negative correlation between soil total phosphorus and Acidobacteria, but soil available phosphorus was also significantly positive related to Acidobacteria, indicating that grazing intensity indirectly affect soil bacteria may be through changing soil properties. This study provides basic data to grazing managements and a theoretical basis to the restoration of degraded grasslands.