The Zoige wetland, located at the northeast of Qinghai-Tibet Plateau, is the largest plateau wetland in China. There have been substantial changes in Zoige wetland due to natural (climate, soil, and vegetation) and anthropogenic (drainage and overgrazing) impacts. The index of biotic integrity is a comprehensive method for assessing the ecological status of wetlands. The ground-dwelling soil animal and wetland plant are the most widely used biological indicators owing to their distinct advantages for wetland ecosystem monitoring, while there is no indicator framework to determine the disturbed status and benefit of returning grazing to wetland of the Zoige area. The aim of this study is to evaluate disturbance status of Zoige wetland using the ground-dwelling soil animal and wetland plant-based index of biotic integrity to providing data for the restoration of Zoige wetland. In July 2018 and July 2019, the ground dwelling soil animal and plant were investigated from 10 typical wetlands of Zoige were chosen, including 4 wetlands which were undisturbed or less disturbed, and 6 wetlands which were disturbed. The ground-dwelling soil animal and plant-based index of biotic integrity in Zoige wetland were determined through a range of index value distribution, discrimination ability, and correlation analysis of 74 candidate indicators, which included 5 metrics of the number of soil animal individuals, the number of meso/micro soil animal individual, the number of ground spiders, Fungivorous form animal and Saprozoic animal, while plant integrity index including 4 metrics of mesophyte, annuals plant, Gentians, ration of annuals/Perennials. Use the quartile method to determine four grades of undisturbed, mild, moderate, severe disturbance in the study area with 95% quantile of all sampling points as the best-expected value and judge the disturbance status of the Zoige swamp wetland. The results showed that the relatively primary wetland was undisturbance, swamp meadow 2 was mild disturbance, long-term low intensity drainage stress area was moderate disturbance, and short-term high intensity drainage stress area was severe disturbance. Among the investigated typical wetland of Zoige, 20% of wetlands were undisturbance, 30% were mild disturbance, 30% were moderate disturbance, and 20% were the severe disturbance. According to the Pearson correlation coefficient analysis, there was a significantly positive correlation between surface soil animal integrity index and plant communities integrity index (r=0.722, P<0.05), indicating that the evaluation results of surface soil animal integrity index and plant integrity index were consistent.