Ecological restoration in regional and landscape scale should aim to improve ecological connectivity and promote the realization of ecological processes. This paper introduces the ecological security pattern and the restoration of ecological connectivity into the field of ecological restoration identification. Taking the extended area of the Guangdong-Hong Kong-Macao Greater Bay Area, the Guangdong-Hong Kong-Macao region as the research area, based on the evaluation of the importance of ecological functions and ecological sensitivity, the minimum cumulative resistance model (MCR) and circuit theory model, the ecological source, ecological corridors, barriers, and ecological pinch points are identified, providing a meso-scale research case on the identification of key areas of land and space ecological restoration. The results indicate (1) The total area of ecological sources in the study area is 52700 km², which are mainly distributed in the main mountain ranges of northern, western and eastern Guangdong, and the central and eastern mountains of the Hong Kong Special Administrative Region, Lantau Island and Hong Kong Island. The ecological sources and ecological corridors together form a circular ecological barrier surrounding the Guangdong-Hong Kong-Macao Greater Bay Area, but there are still protection vacancies in some areas. (2) The areas of ecological barriers and ecological pinch points in the study area are 442.7 and 9.1 km² respectively. The targeted restoration of them can significantly improve ecological connectivity. (3) The ecological sensitive areas is 4302.48 km². There are some ecological degraded areas in important ecological sources such as Qingyun Mountain, Jiulian Mountain and Qiwei Mountain. Restoration of these areas can prevent ecological risks and improve ecological functions. In the future, ecological protection and restoration should strengthen the protection and supervision of important sources and ecological corridors through spatial planning, and carry out zoning and classification restoration of ecological nodes, ecological sensitive areas, and ecological degraded areas. The research results can provide a scientific reference for future ecological protection and restoration work in Guangdong, Hong Kong and Macao.