This study selected Qinghai-Tibet Plateau alpine meadow ecosystem as the research object. Based on the Li-8150 automated soil CO₂ flux system, continuous in-situ observation of soil carbon release at different depths was carried out, and the characteristics of soil carbon release at different depths in sunny and 5 times of continuous rain events were analyzed. Combined with the observation of soil temperature and soil moisture, the effects of rainfall on soil carbon release at different depths and possible mechanisms were analyzed. The results showed that, (1) The soil respiration at 0 cm, 20 cm, 40 cm, and 70 cm all showed obvious single-peak curve, and their daily average soil respiration (±standard deviation) were (3.96±0.89) μmol m^-2 s^-1, (5.09±1.79) μmol m^-2 s^-1, (7.83±1.95) μmol m^-2 s^-1 and (4.43±1.6) μmol m^-2 s^-1. (2) Rainfall significantly increased soil moisture, and inhibited soil carbon release, and the inhibition effects of deep layer was significantly greater than that of surface layer. Among them, the fifth persistent rain event had the most obvious supplementary effect on soil moisture (the largest increment), and its inhibitory effect on soil carbon release was also more significant, even negative flux (i.e., carbon sequestration) appeared in the deep layer. With the increase of soil depth, carbon absorption was observed to be more significant. (3) The relationship between soil carbon release at different depths and soil temperature or soil moisture was correlated with the exponential correlation or quadratic curve. Under sunny conditions, the soil moisture is stable, and the soil temperature is the dominate factor to affect the surface soil respiration. While under rainfall days, soil moisture changed significantly, and became the control of soil carbon release, and its possible mechanism was discussed.