It is of great significance for balancing economic development and environmental protection to study the characteristics of ecosystem services value (ESV) and the trade-offs and synergies between multiple services. Based on the revised equivalence factor table, combined with the land use data from remote sensing interpretation in 2000, 2009 and 2018, the ecosystem services trade-offs and synergies model, sensitivity analysis, spatial autocorrelation and comprehensive index model of land use degree were used to analyze the spatio-temporal evolution characteristics of ESV in Qingyijiang River Basin and the trade-off relationship among nine kinds of ecological services. As the results showed, firstly, in 2000, 2009 and 2018, the ESV of Qingyijiang River Basin was 28.519 billion yuan, 35.126 billion yuan, and 29.468 billion yuan respectively, while the total value showed a trend of first increase and then decrease. Among them, the total ESV decreased by 16.1% from 2009 to 2018, and the ecological services tended to be degraded. Secondly, from 2000 to 2009, the single ecosystem service was dominated by synergy, with the synergy rate of 77.8%. There was a trade-off relationship between food production service and other services in this study. The relationship between ecosystem services in 2009-2018 was almostly same as that in the previous nine years, but the trade-off between food production service and other services was increasing. Thirdly, the sensitivity analysis showed that the coefficient of sensitivity index (CS) values of each period were less than 1, which indicated that the ESV coefficient calculated on the basis of the revised equivalence factor table was consistent with the actual situation of Qingyijiang River Basin, and the calculation results were reliable. Fourth, there was a strongly positive spatial autocorrelation of ESV, but the degree of agglomeration had a downward trend. Bivariate spatial autocorrelation analysis showed that the total service value or individual service value of ecosystem would decrease when the land use degree increased. The results can provide reference information for the optimization and adjustment of land use structure and ecological environment protection in the Qingyijiang River Basin.