Using macrobenthos monitoring data of 3 intertidal zones (Chongming eastern shoal, Nanhui nearshore and Shengshan Island being CM, NH and SS for short) in Changjiang estuary, biodiversity dynamics were evaluated in this study. The results showed that 9 phyla and 57 species of macrobenthos were identified in the intertidal zone of the Changjiang Estuary in 2019, and molluscs, crustacea and polychaeta were the main groups. The CM and NH were muddy intertidal zones, SS was rocky. The average density was 96.4 ind/m², 160.4 ind/m², and 488.9 ind/m² in CM, NH and SS, the average biomass was 68.42 g/m², 45.71 g/m² and 763.72 g/m² in the three areas. There were 3 sections in every area. The diversity, richness and evenness index were in 1.36-3.24, 0.75-2.88 and 0.48-0.77 with every section. The biodiversity of low tide zone was better than that of middle and high tide zone in muddy intertidal zone, but the middle tide zone was the best in rocky intertidal zone. All the macrobenthos were divided into two groups-muddy and rocky. Different species distributed in high, middle and low intertidal zones. Some species in high intertidal zone formed groups with very view in middle intertidal zone and some species in low intertidal zone formed groups with very view in middle intertidal zone. It showed that the sediment environment and tide were the most significant factors to affect community structure of intertidal macrobenthos. During the past 30 a, the species number of macrobenthos undulated in CM and NH, but decreased in SS; the average density and biomass were decreased in 3 areas, which should be related to wetland reclamation, species invasion, human over fishing and pollution.