For a long time, over-exploitation and utilization of natural resources done by human beings have led to a series of serious ecological and environmental problems, such as ecosystem degradation and loss of biodiversity. Nowadays, how to achieve sustainable development of ecosystem has become a joint goal of all countries in the world. Governments of various countries have realized that the establishment of nature reserves can provide long-term protection of important natural ecosystems, natural relics, natural landscapes and the natural resources, ecological functions and cultural values they carry. Nature reserve is one of the core carriers of ecological construction, and it is the main way to protect ecosystem, historical sites, natural landscapes, and biodiversity. Nature reserve provides important ecological support for maintaining national ecological security and achieving sustainable development. The establishment of a large number of nature reserves has gradually highlighted problems of their management methods and models. These problems have begun to restrict the sustainable development of nature reserves, and at the same time, the development of nature reserves is facing major challenges as well as opportunities. How to better protect ecosystem, improve ecosystem service, promote the harmonious development of regions, and realize landsense creation of nature reserves and surrounding areas have become one of the important contents of the study of nature reserve. This article applies the theories of landsenses ecology and the concepts of landsense creation to standardize the management scope of nature reserves. With the goal of better improving the integrity, systematicness and sustainability of ecosystems and from the perspective of better satisfying people's visions and social needs, Dalinor National Nature Reserve, a typical grassland area in the north of China, is used as the case to analyze its landsenses management model through the method of landsense creation. This article clarifies the principles of landsense creation in the reserve, explores the process of landsense creation, and integrates people`s visions, social needs and the reserve`s ultimate goals into management concepts. Through the process of landsense creation, various ecosystem`s services can be improved, enhanced and maintained, and the nature reserve can exert their functions maximumly and sustainably while still meeting the needs of human beings. The process of landsense creation in Dalinor National Nature Reserve has made up for the shortcomings of the original protection system of nature reserve. It innovated protection ideas and improved technology applications, enriched protection concepts and methods, and improved the pertinence and effectiveness of protection strategies.