To understand the effects of trophic interaction and environmental heterogeneity on spatial distribution and functional group characteristics of soil macro-fauna in alpine meadow, we selected four zokor mound density gradients and investigated plant community structure, soil physicochemical properties and functional communities of soil fauna (herbivore, predator, detritivore and omnivore group) to explore the dominant environmental factors which influence the spatial distribution of soil fauna community. Our results showed that the herbivore functional group was the dominant soil fauna group in alpine meadow and the characteristic index of herbivore group was more sensitive to plateau zokor disturbances. The richness and Shannon index of the herbivore group in the extremely severe disturbance intensity were significantly higher than that in the moderate and severe disturbance intensity (P<0.05). The plateau zokor disturbances had a little effect on the stability and similarity of soil fauna. Furthermore, the redundancy analysis indicated that the spatial distribution of soil fauna functional groups was significantly affected by soil temperature, compaction, total nitrogen, total potassium, sedge biomass and plant species evenness(P<0.05), and soil temperature was the dominant factor to influence soil fauna functional groups.