As an important ecological security barrier in China, the Qinghai-Tibet Plateau maintains a large number of important natural ecosystems that are less disturbed by human activities. However, due to the impact of socio-economic development and climate change in recent years, the Qinghai-Tibet Plateau ecosystem is facing the risk of degradation. In order to better protect the Qinghai-Tibet Plateau-the third pole of the earth, the secondly comprehensive scientific investigation of the Qinghai-Tibet Plateau proposed the scientific concept of establishing the Qinghai-Tibet Plateau National Park Group. In order to fully understand the ecosystem integrity status of the Qinghai-Tibet Plateau under the background of human stress and climate change, basing on the understanding of the ecosystem integrity connotation and the ecosystem characteristic of Qinghai-Tibet Plateau, and referring to the ecosystem integrity assessment framework of the Canadian national park, this study builds an indicator system for remote assessment of ecosystem integrity based on the "pattern-quality-function-problem-pressure" framework, and makes a comprehensive assessment of ecosystem integrity of the Qinghai-Tibet Plateau, and then analyzes its characteristics of spatial pattern. The results show that the Qinghai-Tibet Plateau maintains a fair ecosystem integrity. Ecosystem integrity in 3.52% of the region is at excellent level, 7.51% of the region is at good level, 70.71% of the region is at fair level, 18.17% of the region is at poor level, and only 0.10% of the region is at terrible level. From a spatial pattern perspective, the overall ecosystem integrity of the eastern part of the Qinghai-Tibet Plateau is higher than that of the western part. From the perspective of ecosystem integrity protection, there are a large number and wide distribution of areas in the Qinghai-Tibet Plateau suitable for building national parks, and it appears in the pattern of large area and continuous distribution. The current pilot areas of national park system in the Qinghai-Tibet Plateau such as Three-River Source, Qilian Mountain, Giant Panda, and Pudacuo all have high ecosystem integrity. To accelerate the protection of areas with high ecosystem integrity,according to the ecosystem integrity index of the potential construction areas of the Qinghai-Tibet Plateau National Park Cluster, it is recommended to give priority to the construction of the Yarlung Zangbo Grand Canyon National Park, Selincu-Puruogangri National Park, Three Parallel Rivers National Park, Ruoergai National Park, Gongga Mountain National Park and Daocheng Yading National Park; and waiting for the condition is mature, then gradually starting up the construction of the other national parks with landscape and cultural value.