With the rapid development of society and economy, the intensity of human land use is increasing, so the habitat patches tend to be broken and the biologically communicational channels tend to be blocked in some areas. Therefore, it is of great significance for regional sustainable development to study the changes of ecological connectivity and carry out targeted protection and repair. Taking Jiangsu Province as an example, the study uses Morphological Spatial Pattern Analysis method to analyze landscape pattern changes and identify the fragmentation and addition of important ecological sources. On this basis, the article uses the Minimum Cumulative Resistance model to build an ecological network and analyzes the connectivity of ecological spaces. This paper selects overall connectivity index to quantify the relative importance of patches/corridors. Combined with the ecological network structure, the paper analyzes the changes, causes and impacts of corridor connectivity. Finally, the study uses circuit theory and obstacle improvement methods to identify key points of corridor maintenance and dredging, and meanwhile, proposes some suggestions on ecological network protection and restoration. The findings include:(1) from 2000 to 2015, the ecological network of Jiangsu Province showed a pattern of dense west and sparse east. There were 12 ecological node fractures and 5 new ones. The patches in the source area tended to be broken, but the construction of forest nets strengthened the connection between the broken patches. (2) The fragmentation of 6 nodes including the Maling Mountain Scenic Area in Xinyi City has reduced the function of multiple corridor structures in the northern network and needs to be repaired. (3) The corridor connection between the central network and the northern network is improved in some extent, but there is lack of intermediate nodes. (4) The fragmentation of the hilly node of the Dayan Mountain in Danyang weakens the connection between the southern network and the Yangtze River wetland, enhancing the structural importance of the Ningzhen Mountain Range. (5) The connection between the eastern coastal beach node and other nodes is still weak and has not been effectively improved. (6) It is recommended to restore 7 ecological nodes, expand 7 new ecological nodes, build 44 stepping stones, maintain 18 key corridor points, and dredge 5 of them.