A bag cultivation experiment with exogenous cadmium was used to compare the differences in agronomic traits, yield, amino acid and cadmium content of Agaricus blazei strains J37 and J1, which could provide scientific basis for selection and cultivation of Agaricus blazei with low cadmium enrichment. The results showed that the addition of exogenous cadmium had different effects on the body agronomic characteristics of Agaricus blazei strains J37 and J1. The weight, height, cap diameter, cap thickness and stem diameter of individual body for J1 and J 37 had a significantly negative correlation with exogenous cadmium concentration. The correlation between body weight and exogenous cadmium concentration was the largest. The 15 mg/kg cadmium level was the sensitive point for the significant changes in the agronomic characteristics of them. The individual body weight of J37 was 10.9%-36.6% higher than that of J1 under different cadmium levels, which indicated that J37 had strong cadmium tolerance. The yield, amino acid and cadmium content of the bodies of J37 and J1 strains decreased with the increase of cultivation numbers. The yield and amino acid content of them decreased with the increase of exogenous cadmium level, but the cadmium content increased with the increase of exogenous cadmium level. When the exogenous cadmium level was higher than 15 mg/kg, the yield of J1 and J37 decreased by 23.4% and 13.4% than that of no cadmium treatment, respectively. The cadmium content of them increased by 51.5% and 13.7%, respectively, which of J1 was close to food hygiene standards. When the level of exogenous cadmium reached 35 mg/kg, the yield of J1 and J37 decreased by 45% and 32%, and the cadmium content of them increased by 75% and 68%, respectively. The amino acid content in the fruit bodies of J37 with cadmium treatments (0, 15 and 35 mg/kg) increased by 20.1%, 16.6%, and 16.1% than that of J1, respectively, and the difference between them reached a significant level (P<0.05). With the increase of harvest tides, the amino acids contents of J1 and J37 gradually decreased, but the difference between the tides was not significant. The results showed that J37 was relatively resistant to cadmium stress than J1, but the cadmium content in the culture material should be controlled below 15 mg/kg, which could help to ensure that the fruit body products meet the food hygiene standards.