Conducting ecological benefit assessment is of great significance for promoting ecological benefits to be included in economic and social evaluation system and pushing the construction of ecological civilization. Ecological benefits refer to the beneficial effects of natural ecosystems on human production, living conditions and environmental conditions. Gross Ecosystem Product (GEP) is a measure of the aggregate monetary value of ecosystem goods and services in a given region in an accounting period, which can reflect the ecological benefits of specific area. This research proposed the framework of GEP accounting for ecological benefits assessment, established the accounting indicator system and methods. On the basis of remote sensing data and statistical data, this research took Qinghai Province as a case to measure the GEP for ecological benefits assessment, and analyzed the stakeholders. The research results show that the GEP of Qinghai Province in 2015 was 46.42 billion Yuan, and increased by 32.6% since 2000. More than 80% of these benefits accrue regionally to other provinces out of Qinghai Province. GEP can provide visibility and give prominence to the values of nature and their contributions to human well-being with available data and methods, as well as contribute to ecological benefits assessment, which can be used as an important supplement of GDP in assessment of policy effects and ecological protection effectiveness, also serve as the reference for the establishment of market-oriented and diversified ecological compensation mechanism and the transformation from "lucid waters and lush mountains" to "golden and silver mountains", providing important information for decision makers.