Abstract:Amphiprion ocellaris is one of the most popular marine ornamental fish species. Its growth performance has been degraded during artificial culture, so selective breeding programs aiming to improve growth is necessary. Some individuals of A. ocellaris may be starved for a relatively long period by some factors during artificial culturing. However, it remains unclear whether starvation has an effect on selecting suitable breeding index. In this study, multivariate path analysis was used to describe the relationship between body weight and morphological characteristics of juvenile A. ocellaris after 7 days of starvation (starvation group) and non-starvation (control group). Body weight (g) and 13 morphological characters (mm) such as total length (L1), standard length (L2), body depth (L3), head length (L4), head depth (L5), snout length (L6), caudal-peduncle length (L7), caudal-peduncle depth (L8), pre dorsal length (L9), pre pectoral length (L10), pre pelvic length (L11), pre anal length (L12), and eye diameter(L13) were measured accurately. The statistical methods such as correlation coefficient, determination coefficient, path coefficient and regression equation were used to analyze the effects of morphological characteristics on body weight under two conditions. The results showed that there was a significant correlation between the 14 personality traits of the two experimental groups (P<0.01); after removing the collinearity effect, the path analysis selected four morphological traits of the control group that had a significant impact on the body weight. The direct effect was ranked from large to small in the order of L3 > L2 > L6 > L5, and the indirect effect was ranked from large to small L5 > L6 > L2 > L3, and the order of independent determination coefficient was L3 > L2 > L5 > L6. For the starvation group, 5 morphological characters were selected, and the direct effect was L3>L2>L12>L6>L7, the indirect effect was L7> L12 > L2 > L6 > L3, and the independent determination coefficient was L3 > L2 > L12 > L7 > L6. The maximum collaborative coefficient of determination to body weight was body height and body length both in the control group and the starvation group. For the control group and the starvation group, the multiple regression equations of body weight and morphological characters were W=-0.819+0.106L3+0.027L2+0.006L6-0.050L5, R2=0.941; W=-0.778+0.078L3+0.016L12+0.015L2-0.055L7+0.018L6, R2=0.930. The results showed that starvation affected the correlation between morphological traits and body weight, but body depth, body length and snout length were the key factors affecting body weight under starvation and non-starvation conditions, among which body depth was the most important factor. The results could provide theoretical support for the determination of breeding indexes in the selection and breeding work of A. ocellaris.